Description
Intended Use The Chlamydia Trachomatis IgG kit is intended for the detection of IgG antibody to Chlamydia Trachomatis in human serum or plasma. For research use only, not for use in diagnostic procedures.
PRINCIPLE OF THE TEST Diluted serum is added to wells coated with purified antigen. IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the unknown